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详细说明
概述
产品名称Anti-LLGL1抗体[EPR18899]
描述
兔单克隆抗体[EPR18899] to LLGL1
经测试应用IHC-P,WB,ICC/IF,IP
种属反应性
与反应: Human
免疫原
Recombinant fragment within Human LLGL1 aa 1-400. The exact sequence is proprietary.
Database link: Q15334Run BLAST with
Run BLAST with 
阳性对照
WB: HepG2, SW480, A431 and 293 whole cell lysates; Human fetal liver, fetal heart and fetal kidney lysates. IHC-P: Human kidney and stomach tissues. ICC/IF: HepG2 and SW480 cells. IP: HepG2 whole cell lysate.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol纯度Protein A purified
克隆单克隆
克隆编号EPR18899
同种型IgG
研究领域
Stem Cells
Neural Stem Cells
Surface Molecules
Stem Cells
Neural Stem Cells
Intracellular
Stem Cells
Signaling Pathways
Notch
Cytoplasmic
Stem Cells
Signaling Pathways
Notch
Surface Molecules
Neuroscience
Neurology process
Notch Pathway
Anti-LLGL1 antibody [EPR18899] 图像
![Western blot - Anti-LLGL1 antibody [EPR18899] (ab183021)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183021-WB.jpg)
Western blot - Anti-LLGL1 antibody [EPR18899] (ab183021)
All lanes : Anti-LLGL1 antibody [EPR18899] (ab183021) at 1/5000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : A459 cell lysate
Lane 5 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (HRP goat anti-rabbit IgG (H+L))
Predicted band size : 115 kDa
Observed band size : 115 kDa
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST![Western blot - Anti-LLGL1 antibody [EPR18899] (ab183021)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183021WBb.jpg)
Western blot - Anti-LLGL1 antibody [EPR18899] (ab183021)
All lanes : Anti-LLGL1 antibody [EPR18899] (ab183021) at 1/2000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size : 115 kDa
Observed band size : 115 kDa
Blocking/dilution buffer: 5% NFDM/TBST.
Exposure time Lane 1/2: 3 minutes; Lane 3: 15 seconds.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LLGL1 antibody [EPR18899] (ab183021)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183021HCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LLGL1 antibody [EPR18899] (ab183021)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling LLGL1 with ab183021 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human kidney is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LLGL1 antibody [EPR18899] (ab183021)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183021HCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LLGL1 antibody [EPR18899] (ab183021)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling LLGL1 with ab183021 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human stomach is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-LLGL1 antibody [EPR18899] (ab183021)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183021IFa.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LLGL1 antibody [EPR18899] (ab183021)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling LLGL1 with ab183021 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [EPR18899]-Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab183021 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.![Immunocytochemistry/ Immunofluorescence - Anti-LLGL1 antibody [EPR18899] (ab183021)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183021IFb.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LLGL1 antibody [EPR18899] (ab183021)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (Human colorectal adenocarcinoma cell line) cells labeling LLGL1 with ab183021 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SW480 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [EPR18899]-Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab183021 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.![Immunoprecipitation - Anti-LLGL1 antibody [EPR18899] (ab183021)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183021-ip.jpg)
Immunoprecipitation - Anti-LLGL1 antibody [EPR18899] (ab183021)
LLGL1was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab183021 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab183021 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.
Lane 1: HepG2 whole cell lysate 10µg (Input).
Lane 2: ab183021 IP in HepG2 whole cell lysate.
Lane 3: Rabbit IgG, monoclonal [EPR18899] - Isotype Control (ab172730) instead of ab183021 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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