• Species Reactivity

  Mouse

• Specificity

  Detects mouse Stella/Dppa3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human Stella is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant mouse Stella/Dppa3    
  Met1-Asn150    
  Accession # Q8QZY3

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse Stella/Dppa3

• 
  

• Immunohistochemistry

  5-15 µg/mL

  Perfusion fixed frozen sections of mouse ovary

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Stella/Dppa3

Stella, also known as Dppa3 (Developmental pluripotency-associated 3) and PGC7 (primordial germ cell 7) is expressed in primordial germ cells, oocytes, preimplantation embryos, and pluripotent cells. Although the fuction of Stella is not known, the expression pattern of the protein suggests a role in the maintenance of cell pluripotency.

• Long Name:

  Developmental Pluripotency-associated Protein 3

• Entrez Gene IDs:

  359787 (Human); 73708 (Mouse)

• Alternate Names:

  developmental pluripotency associated 3; DPPA3 developmental pluripotency associated 3; Dppa3; Stella