• Species Reactivity

  Mouse

• Specificity

  Detects mouse CD300b/LMIR5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human LMIR5 is observed and less than 5% cross‑reactivity with recombinant human (rh) LMIR1, rhLMIR2, recombinant mouse LMIR3, and rhLMIR6 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse CD300b/LMIR5    
  Ile18-Tyr157    
  Accession # Q3U497

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse CD300b/LMIR5

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300b/LMIR5

The LMIR members, alternatively known as CLM (CMRF-35 like molecules) multigene family members, are closely mapped in the distal region of mouse chromosome 11 and are expressed widely in leukocytes. Their encoded proteins are type I membrane proteins that contain a single extracellular Ig-like domain. These proteins either have cytoplasmic ITIM motifs or can interact with ITAM motif-bearing molecules through a characteristic transmembrane domain containing positively charged amino acid residues.

• Long Name:

  Leukocyte Mono Ig-like Receptor 5

• Entrez Gene IDs:

  124599 (Human); 217304 (Mouse)

• Alternate Names:

  CD300 antigen like family member B; CD300 antigen-like family member B; CD300 molecule-like family member b; CD300b antigen; CD300b; CD300LB; CLM7; CLM-7; CLM7IREM3CD300 molecule like family member b; CMRF35A2; CMRF35-A2; CMRF35-like molecule 7; EC 1.13.11.6; EC 6.3.4.3; Gm253; Immune receptor expressed on myeloid cells 3; IREM-3; Leukocyte mono-Ig-like receptor 5; LMIR5; TREM-5; TREM5CD300b; Triggering receptor expressed on myeloid cells 5