概述

• 产品名称Anti-PARK7/DJ1抗体[EP2815Y]
     

• 描述

  兔单克隆抗体[EP2815Y] to PARK7/DJ1

• 经测试应用WB,IP,IHC-P,ICC,Flow Cyt,ICC/IF

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide corresponding to residues near the N-terminus of human PARK7/DJ1.

• 阳性对照    

• WB: Jurkat, HeLa, NIH3T3 or 293T cell lysate. IHC-P: Brain tissue. IF/ICC: PANC-1 cell line

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

   

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Alternative versions available:

  Anti-PARK7/DJ1 antibody (Alexa Fluor^® 488) [EP2815Y] (ab203989)

  Anti-PARK7/DJ1 antibody (Alexa Fluor^® 647) [EP2815Y] (ab204009)

  Anti-PARK7/DJ1 antibody (HRP) [EP2815Y] (ab204010)

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EP2815Y

• 同种型IgG

• 研究领域

• Metabolism

• Pathways and Processes

• Redox metabolism

• Oxidative stress

• Cancer

• Signal transduction

• G protein signaling

• Small G proteins

• Other

• Epigenetics and Nuclear Signaling

• Chromatin Binding Proteins

• DNA / RNA binding

• Signal Transduction

• Signaling Pathway

• G Protein Signaling

• Small G Proteins

• Regulators

• Neuroscience

• Neurology process

• Neurodegenerative disease

• Parkinson's disease

• Parkin / PARK

Anti-PARK7/DJ1 antibody [EP2815Y] 图像

• 

  Western blot - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)          

     
  
  Predicted band size : 20 kDa
     

  Lane 1: Wild-type HAP1 cell lysate (20 µg)
  Lane 2: PARK7/DJ1 knockout HAP1 cell lysate (20 µg)
  Lane 3: HeLa cell lysate (20 µg)
  Lane 4: Human brain tissue lysate (20 µg)
  Lanes 1 - 4: Merged signal (red and green). Green - ab76008 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
  ab76008 was shown to specifically react with PARK/DJ1 when PARK/DJ1 knockout samples were used. Wild-type and PARK/DJ1 knockout samples were subjected to SDS-PAGE. ab76008 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

   

• 

  Flow Cytometry - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)          

     Overlay histogram showing HepG2 cells stained with ab76008 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76008, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008)          

     

  ICC/IF image of ab76008 stained PANC-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76008, 1/50 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

   

• 

  Western blot - PARK7/DJ1 antibody [EP2815Y] (ab76008)          

     All lanes : Anti-PARK7/DJ1 antibody [EP2815Y] (ab76008) at 1/20000 dilution
  
  Lane 1 : Jurkat cell lysate
  Lane 2 : HeLa cell lysate
  Lane 3 : NIH3T3 cell lysate
  Lane 4 : 293T cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat anti-rabbit HRP at 1/1000 dilution
  
  Predicted band size : 20 kDa
  Observed band size : 23 kDa (why is the actual band size different from the predicted?)
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PARK7/DJ1 antibody [EP2815Y] (ab76008)          

     ab76008, at 1/250 dilution, staining PARK7/DJ1 in human brain by immunohistochemistry using paraffin-embedded tissue.