概述

• 产品名称Anti-PARK7/DJ1抗体[EP2816Y]
     

• 描述

  兔单克隆抗体[EP2816Y] to PARK7/DJ1

• 经测试应用WB,IHC-P,ICC,Flow Cyt

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide derived from an internal region of human DJ1

• 阳性对照    

• TF-1 cell lysate Jurkat cell lysate HeLa cell lysate Human brain tissue

• 常规说明

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EP2816Y

• 同种型IgG

• 研究领域

• Metabolism

• Pathways and Processes

• Redox metabolism

• Oxidative stress

• Cancer

• Oncoproteins/suppressors

• Oncoproteins

• Other

• Cancer

• Signal transduction

• G protein signaling

• Small G proteins

• Other

• Cell Biology

• Other Antibodies

• Oxidative Stress

• Epigenetics and Nuclear Signaling

• Chromatin Binding Proteins

• DNA / RNA binding

• Epigenetics and Nuclear Signaling

• Transcription

• Other factors

• Epigenetics and Nuclear Signaling

• Nuclear Signaling Pathways

• Nuclear Receptors

• Testosterone

• Signal Transduction

• Protein Trafficking

• Chaperones

• Other Chaperones

• Signal Transduction

• Signaling Pathway

• Nuclear Signaling

• Nuclear Hormone Receptors

• Testosterone

• Signal Transduction

• Signaling Pathway

• G Protein Signaling

• Small G Proteins

• Regulators

• <a javascript:void(0)>Neuroscience

• Neurology process

• Neurodegenerative disease

• Parkinson's disease

• Parkin / PARK

Anti-PARK7/DJ1 antibody [EP2816Y] 图像

• 

  Western blot - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)          

     
  
  Predicted band size : 20 kDa
     

  Lane 1: Wild-type HAP1 cell lysate (20 µg)
  Lane 2: PARK/DJ1 knockout HAP1 cell lysate (20 µg)
  Lane 3: HeLa cell lysate (20 µg)
  Lane 4: Human brain tissue lysate (20 µg)
  Lanes 1 - 4: Merged signal (red and green). Green - ab76241 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
  ab76241 was shown to specifically react with PARK7/DJ1 when PARK7/DJ1 knockout samples were used. Wild-type and PARK7/DJ1 knockout samples were subjected to SDS-PAGE. ab76241 and ab8245 (loading control to PARK7/DJ1) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

   

• 

  Flow Cytometry - Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241)          

     Overlay histogram showing Jurkat cells stained with ab76241 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76241, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.  

• 

  Western blot - DJ1 antibody [EP2816Y] (ab76241)          

     All lanes : Anti-PARK7/DJ1 antibody [EP2816Y] (ab76241) at 1/20000 dilution
  
  Lane 1 : TF-1 cell lysate
  Lane 2 : Jurkat cell lysate
  Lane 3 : HeLa cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/1000 dilution
  
  Predicted band size : 20 kDa
  Observed band size : 24 kDa (why is the actual band size different from the predicted?)
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DJ1 antibody [EP2816Y] (ab76241)          

     Immunohistochemical analysis of paraffin-embedded human brain tissue using ab76241 at 1/100 dilution.