详细说明
概述
产品名称Anti-PKC alpha (phospho T638)抗体[E195]
描述
兔单克隆抗体[E195] to PKC alpha (phospho T638)
特异性The antibody only detects PKC alpha phosphorylated on Threonine 638.
经测试应用WB,IHC-P,ICC/IF,IP
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Chicken, Cow免疫原
A synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKC alpha.
阳性对照
293 cell lysate and normal brain tissue.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A trial size is available to purchase for this antibody.
性能
形式Liquid
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
纯度Protein A purified
克隆单克隆
克隆编号E195
同种型IgG
研究领域
Cardiovascular
Heart
Hypertrophy
Other
Cancer
Signal transduction
Protein phosphorylation
Serine/threonine kinases
Other
<a javascript:void(0)>Signal Transduction
Protein Phosphorylation
Ser / Thr Kinases
PKC
Anti-PKC alpha (phospho T638) antibody [E195] 图像
Western blot - PKC alpha (phospho T638) antibody [E195] (ab32502)
All lanes : Anti-PKC alpha (phospho T638) antibody [E195] (ab32502) at 1/20000 dilution
Lane 1 : 293 cell lysate, untreated
Lane 2 : 293 cell lysate, treated with TPA.
Predicted band size : 77 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - PKC alpha (phospho T638) antibody [E195] (ab32502)
Immunohistochemical staining of untreated paraffin-embedded normal brain tissue using ab32502 at 1/250 dilution.
Immunohistochemistry (Paraffin-embedded sections) - PKC alpha (phospho T638) antibody [E195] (ab32502)
Immunohistochemical staining of Phosphatase-treated paraffin-embedded normal brain tissue using ab32502 at 1/250 dilution