概述

• 产品名称Anti-PKC alpha抗体[Y124]
     

• 描述

  兔单克隆抗体[Y124] to PKC alpha

• 经测试应用WB,IHC-P,ICC/IF,Flow Cyt,IP,IHC-Fr

• 种属反应性

  与反应: Mouse, Rat, Human, Pig, Goldfish    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PKC alpha aa 650 to the C-terminus (C terminal). The exact sequence is proprietary.

• 阳性对照    

• WB: 293 cell lysate. IHC-P: Human lung carcinoma. IF: HeLa cells.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

   

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  A trial size is available to purchase for this antibody.

  Alternative versions available:

  Anti-PKC alpha antibody (Alexa Fluor^® 488) [Y124] (ab204633)
  Anti-PKC alpha antibody (Alexa Fluor^® 647) [Y124] (ab204634)

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 
  

• 克隆单克隆

• 克隆编号Y124

• 同种型IgG

• 研究领域

• Cardiovascular

• Heart

• Hypertrophy

• Other

• Cancer

• Signal transduction

• Protein phosphorylation

• Serine/threonine kinases

• Other

• Signal Transduction

• Protein Phosphorylation

• Ser / Thr Kinases

• PKC

Anti-PKC alpha antibody [Y124] 图像

• 

  Western blot - Anti-PKC alpha antibody [Y124] (ab32376)          

     
  
  Predicted band size : 77 kDa
     

  Lane 1: Wild-type HAP1 cell lysate (20 µg)
  Lane 2: PKC alpha knockout HAP1 cell lysate (20 µg)
  Lane 3: K562 cell lysate (20 µg)
  Lane 4: HEK293 cell lysate (20 µg)
  Lanes 1 - 4: Merged signal (red and green). Green - ab32376 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa.
  ab32376 was shown to specifically react with PKC alpha when PKC alpha knockout samples were used. Wild-type and PKC alpha knockout samples were subjected to SDS-PAGE. ab32376 and ab8245 (loading control to GAPDH) were diluted 1/5000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

   

• 

  Western blot - PKC alpha antibody [Y124] (ab32376)          

     Anti-PKC alpha antibody [Y124] (ab32376) at 1/10000 dilution + 293 cell lysate
  
  Predicted band size : 77 kDa
  Observed band size : 75 kDa (why is the actual band size different from the predicted?)
   

• 

  Immunohistochemistry (Paraffin-embedded sections) - PKC alpha antibody [Y124] (ab32376)          

     Ab32376, at a 1/250 dilution, staining PKC alpha in paraffin embedded human lung carcinoma tissue sections by Immunohistochemistry.     Ab32376, at a 1/250 dilution, staining PKC alpha in paraffin embedded human lung carcinoma tissue sections by Immunohistochemistry.   

• 

  Immunocytochemistry/ Immunofluorescence - PKC alpha antibody [Y124] (ab32376)          

     Ab32376, at a 1/250 dilution, staining PKC alpha in HeLa cells by Immunofluorescence.   

• 

  Immunocytochemistry - PKC alpha antibody [Y124] (ab32376)          

  See Abreview

• 

  Flow Cytometry-PKC alpha antibody [Y124](ab32376)          

     Overlay histogram showing HeLa cells stained with ab32376 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32376, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [Y124] (ab32376)      Image from Hao F et al., J Biol Chem. 2011 May 20;286(20):18104-17. Epub 2011 Mar 18. Fig 1.; doi: 10.1074/jbc.M110.208488; May 20, 2011 The Journal of Biological Chemistry, 286, 18104-18117.    

     Immunohistochemical analysis of mouse small intestine tissue, staining PKC alpha with ab32376.