• Species Reactivity

  Human

• Specificity

  Detects human MAO-A in direct ELISAs and Western blots. 

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  S. frugiperda insect ovarian cell line     Sf21-derived recombinant human MAO-A    
  Met1-Ser497 (predicted)    
  Accession # P21397

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAO-A

MAO-A (Monoamine oxidase type A and Amine oxidase [flavin-containing] A)  is a 60 kDa (predicted) member of the flavin monoamine oxidase family of proteins.  MAO- A is an enzyme that degrades amine neurotransmitters, such as dopamine, norepinephrine, and serotonin. It localizes to the outer mitochondrial membrane. Mutation in this gene results in monoamine oxidase deficiency, or Brunner syndrome, a form of X-linked non-dysmorphic mild mental retardation. Male patients are affected by borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior. Over aa 1-497, human MOA-A shares 88% aa sequence identity with mouse MOA-A.

• Long Name:

  Monoamine Oxidase A

• Entrez Gene IDs:

  4128 (Human); 17161 (Mouse); 29253 (Rat)

• Alternate Names:

  amine oxidase [flavin-containing] A; EC 1.4.3; EC 1.4.3.4; MAOA; MAO-A; monoamine oxidase A; Monoamine oxidase type A