• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse Caspase-2 precursor and the small Caspase-2 subunit that is generated during proteolytic activation in Western blots.

• Source

  Polyclonal Rabbit IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  KLH-coupled mouse Caspase-2 synthetic peptide    
  KEREGYAPGTEFHRC

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.8 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspase-2

Caspase-2 (Cysteine-aspartic acid protease 2/Casp2; also NEDD2 and ICH-1) is a 30‑32 kDa member of the peptidase C14A/IL-1 beta -converting family of enzymes (1‑3). It is widely expressed and is an integral component of the apoptotic cascade. Based on the length of its prodomain, Caspase‑2 has been considered to be an initiator caspase. However, studies have shown that other caspases (such as Casp3) activate procaspase 2, and Caspase‑2 likely acts on key cellular molecules such as BID, Golgin 160 and DFF45/ICAD (2, 4, 5). Thus, Caspase‑2 is perhaps more likely to be a specialized executioner caspase. Human procaspase‑2 is a 48‑51 kDa, 452 amino acid (aa) protein (4‑7). It is known to exist as a disulfide‑linked homodimer via covalent linkage at Cys436 (2, 5). But this dimeric state may not be sufficient for (auto)activation. Actual activation may occur following oligomerization within the context of activating platforms such as DISC (death‑inducing signaling complex) or the PIDDosome (8‑10). Initially, procaspase‑2 undergoes proteolytic cleavage to generate an N‑terminal 333 aa p34/34 kDa subunit, and a 119 aa C‑terminal p14/14 kDa subunit (5). The p34 and p14 subunits are further processed to generate the prodomain (aa 1‑169), plus the mature p18 (aa 170‑333) and p12 (aa 348‑452) subunits (4‑6). Notably, each p18:p12 noncovalent heterodimer demonstrates proteolytic activity around a catalytic site at aa 318‑322, and, due to an nuclear localization signal within the prodomain, may be found in either nucleus or cytoplasm (11, 12). There are multiple potential isoform variants. Individually, or in combination, there is an alternative start site at Met18, a substitution of two aa for aa 107‑452, a second substitution of 14 aa for aa 309‑322, and a third substitution of 22 aa for aa 323‑452 (6, 7, 13). The human and mouse procaspase 2 precursors are 90% aa identical, with the majority of differences lying in the prodomain.

• References:

1. Chowdhury, I. et al. (2008) Comp. Biochem. Physiol. B 151:10.

2. Krumschnabel, G. et al. (2009) Cell Death Differ.16:195.

3. Kitevska, T. et al. (2009) Apoptosis 14:829.

4. Paroni, G. et al. (2001) J. Biol. Chem. 276:21907.

5. Li, H. et al. (1997) J. Biol. Chem. 272:21010.

6. SwissProt. Accession # P42575.

7. Wang, L. et al. (1994) Cell 78:739.

8. Chang, D.W. et al. (2003) J. Biol. Chem. 278:16466.

9. Olsson, M. et al. (2009) Oncogene 28:1949.

10. Tinel, A. & J. Tschopp (2004) Science 304:843.

11. Schweizer, A. et al. (2003) J. Biol. Chem. 278:42441.

12. Colussi, P.A. et al. (1998) J. Biol. Chem. 273:24535.

13. Droin, N. et al. (2000) Cancer Res. 60:7039.

• Entrez Gene IDs:

  835 (Human); 12366 (Mouse)

• Alternate Names:

  caspase-2; CASP2; CASP-2; caspase 2, apoptosis-related cysteine peptidase; Caspase2; Caspase-2; ICH1; ICH-1; NEDD2; NEDD-2; PPP1R57