详细说明
概述
产品名称Anti-PPIC抗体[EPR15355] - C-terminal
描述
兔单克隆抗体[EPR15355] to PPIC - C-terminal
经测试应用WB,IHC-P,Flow Cyt
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PPIC aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: P45877阳性对照
Human lung carcinoma and mouse cardiac muscle tissues; HeLa, U87-MG and Human fetal kidney lysates; U87-MG cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR15355
同种型IgG
研究领域
Signal Transduction
Protein Trafficking
Chaperones
Other Chaperones
Immunology
Immune Interventions
Transplantation
Anti-PPIC antibody [EPR15355] - C-terminal 图像
![Western blot - Anti-PPIC [EPR15355] antibody (ab184552)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184552552.JPG)
Western blot - Anti-PPIC [EPR15355] antibody (ab184552)
All lanes : Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/10000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 23 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC [EPR15355] antibody (ab184552)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184552523.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC [EPR15355] antibody (ab184552)
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling PPIC with ab184552 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
![Western blot - Anti-PPIC [EPR15355] antibody (ab184552)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184552522.JPG)
Western blot - Anti-PPIC [EPR15355] antibody (ab184552)
Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/50000 dilution + HeLa cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 23 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC [EPR15355] antibody (ab184552)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184552524.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPIC [EPR15355] antibody (ab184552)
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling PPIC with ab184552 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
![Flow Cytometry - Anti-PPIC [EPR15355] antibody (ab184552)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab184552525.JPG)
Flow Cytometry - Anti-PPIC [EPR15355] antibody (ab184552)
Flow cytometric analysis of U87-MG cells (2% paraformaldehyde-fixed) labeling PPIC with ab184552 at 1/250 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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