• Species Reactivity

  Human

• Specificity

  Detects human NT-4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) NT-3 and rhBDNF is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human NT-4    
  Gly81-Ala210    
  Accession # P34130

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Human NT‑4 (Catalog # )

• 
  

• Immunohistochemistry

  5-15 µg/mL

  Immersion fixed paraffin-embedded sections of human brain (cortex)

• 
  

• Neutralization

  Measured by its ability to neutralize NT‑4-induced proliferation in BaF‑TrKB‑BD mouse pro‑B cell line transfected with TrkB. The Neutralization Dose (ND    ₅₀) is typically 0.4-2 µg/mL in the presence of 40 ng/mL Recombinant Human NT‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NT-4

Neurotrophin-4 (NT-4), also known as NT-5, is a member of the NGF family of neuronal and epithelial growth factors. Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds (1‑3). The human NT-4 cDNA encodes a 210 amino acid (aa) precursor that includes a 24 aa signal sequence, a 56 aa propeptide, and a 130 aa mature protein (4, 5). NT‑4 is synthesized as a 28 kDa prepropeptide that is proteolytically processed to generate the mature protein. Mature human NT-4 shares 48‑52% aa sequence identity with human beta-NGF, BDNF, and NT-3. It shares 91% and 95% aa sequence identity with mouse and rat NT-4/5, respectively. The mature protein is secreted as a homodimer and can also form heterodimers with BDNF or NT-3 (6). NT-4 binds and induces receptor dimerization and activation of TrkB (4, 7). NT-4 promotes the development and survival of selected peripheral and CNS neurons (8‑10). BDNF, which also activates TrkB, overlaps with many but not all NT-4 functions, a distinction that is likely due to differences in expression patterns (8‑10). NT-4 induced TrkB signaling augments NMDA receptor activity and increases neuronal sensitivity to excitotoxic cell death (11). It also promotes the proliferation of keratinocytes and accelerates hair follicle regression during the follicular cycle (12, 13). NT-4 is secreted by activated T cells and granulocytes at sites of inflammation where it contributes to tissue regeneration (14‑16).

• References:

1. Lessmann, V. et al. (2003) Prog. Neurobiol. 69:341.

2. Tabakman, R. et al. (2004) Prog. Brain Res. 146:387.

3. Botchkarev, V.A. et al. (2004) Prog. Brain Res. 146:493.

4. Ip, N.Y. et al. (1992) Proc. Natl. Acad. Sci. 89:3060.

5. Berkemeier, L.R. et al. (1991) Neuron 7:857.

6. Radziejewski, C. and R.C. Robinson (1993) Biochemistry 32:13350.

7. Vesa, J. et al. (2000) J. Biol. Chem. 275:24414.

8. Davies, A.M. et al. (1993) J. Neurosci. 13:4961.

9. Stucky, C.L. et al. (1998) J. Neurosci. 18:7040.

10. Fan, G. et al. (2000) Nat. Neurosci. 3:350.

11. Choi, S.Y. et al. (2004) J. Neurochem. 88:708.

12. Botchkarev, V.A. et al. (1999) Lab. Invest. 79:557.

13. Botchkarev, V.A. et al. (1999) FASEB J. 13:395.

14. Laurenzi, M.A. et al. (1998) J. Leukoc. Biol. 64:228.

15. Moalem, G. et al. (2000) J. Autoimmun. 15:331.

16. Nassenstein, C. et al. (2003) J. Exp. Med. 198:455.

• Long Name:

  Neurotrophin 4

• Entrez Gene IDs:

  4909 (Human)

• Alternate Names:

  GLC10; GLC1O; neurotrophic factor 4; neurotrophic factor 5; neurotrophin 4; neurotrophin 5 (neurotrophin 4/5); neurotrophin-4; neurotrophin-5; Neutrophic factor 4; NT4; NT-4; NT-4/5; NT-5; NTF4; NTF5; NTF5NT5