• Species Reactivity

  Human, Mouse

• Specificity

  Detects endogenous human and mouse NF kappa B1 in Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human NF kappa B1    
  Pro317-Asp437    
  Accession # NP_003989

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.5 µg/mL

  See below

• 
  

• Chromatin Immunoprecipitation (ChIP)

  5 µg/5 x 10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFkB1

Nuclear Factor kappa B1 (NF kappa B1 or NF kappa B p50) is a member of the rel/NF kappa B family of transcription factors. NF kappa B1 dimerizes with other members of the rel/NF kappa B family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes.

• Long Name:

  Nuclear Factor Kappa-B, Subunit 1

• Entrez Gene IDs:

  4790 (Human); 18033 (Mouse)

• Alternate Names:

  DNA-binding factor KBF1; EBP-1; NF-kappa-B; NFkB1; NF-kB1; NFKB-p105; Nuclear factor NF-kappa-B p105 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; p105