详细说明
- Species ReactivityHuman
- SpecificityDetects human BRCA1 N-Terminus in Western blots.
- SourcePolyclonal Goat IgG
- PurificationAntigen Affinity-purified
- ImmunogenE. coli-derived recombinant human BRCA1 N-Terminus
Met1-Gly200
Accession # P38398 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Recommended
ConcentrationSample
- Western Blot1 µg/mLSee below
- Immunohistochemistry5-15 µg/mLSee below
- Immunoprecipitation3 µg/500 µg cell lysateU2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line,
Immunohistochemistry | BRCA1 in Human Breast Cancer Tissue. BRCA1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 5 µg/mL Goat Anti-Human BRCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2210) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ) and counterstained with hematoxylin (blue). View our protocol for . |
Western Blot | Detection of Human BRCA1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-human BRCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2210) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # ). A specific band was detected for BRCA1 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
- ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The BRCA1 (BReast CAncer gene 1) tumor suppressor protein has many reported functions. In addition to mediating signal transduction in DNA damage and repair responses, BRCA1 regulates transcriptional activity and assists in the preservation of chromosomal stability. BRCA1 is one of the first proteins recruited to sites of DNA double-strand breaks, and serves as part of a scaffold for assembling other DNA damage response or repair factors.
- Long Name:Breast Cancer 1
- Entrez Gene IDs:672 (Human); 12189 (Mouse); 497672 (Rat)
- Alternate Names:BRCA1; BRCAI; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein; breast cancer 1, early onset; breast cancer type 1 susceptibility protein; EC 6.3.2; EC 6.3.2.-; IRIS; PNCA4; PSCP; subunit 1