详细说明
概述
产品名称Anti-PCK2抗体[EPR14224] - C-terminal
描述
兔单克隆抗体[EPR14224] to PCK2 - C-terminal
经测试应用WB,IHC-P,Flow Cyt,ICC/IF
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide within Human PCK2 aa 550 to the C-terminus. The exact sequence is proprietary.
Database link: Q16822Run BLAST with
Run BLAST with 
阳性对照
HepG2, MCF7, HeLa, mouse brain, mouse kidney, rat brain, rat kidney and rat spleen lysates; Human liver tissue; HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR14224
同种型IgG
研究领域
Metabolism
Types of disease
Obesity
Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Cholesterol Metabolism
Metabolism
Pathways and Processes
Metabolic signaling pathways
Carbohydrate metabolism
Metabolism
Pathways and Processes
Mitochondrial Metabolism
Mitochondrial markers
Cancer
Cancer Metabolism
Metabolic signaling pathway
Metabolism of carbohydrates
Signal Transduction
Metabolism
Mitochondrial
Signal Transduction
Protein Phosphorylation
Ser / Thr Kinases
PKC
Cardiovascular
Lipids / Lipoproteins
Lipid Metabolism
Cholesterol Metabolism
Anti-PCK2 antibody [EPR14224] - C-terminal 图像
![Western blot - Anti-PCK2 [EPR14224] antibody (ab187145)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab187145145.jpg)
Western blot - Anti-PCK2 [EPR14224] antibody (ab187145)
Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145) at 1/10000 dilution + HepG2 lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 71 kDa
![Western blot - Anti-PCK2 [EPR14224] antibody (ab187145)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18714545b.jpg)
Western blot - Anti-PCK2 [EPR14224] antibody (ab187145)
All lanes : Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145) at 1/1000 dilution
Lane 1 : MCF7 lysate
Lane 2 : Hela lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 71 kDa
![Western blot - Anti-PCK2 [EPR14224] antibody (ab187145)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18714545c.jpg)
Western blot - Anti-PCK2 [EPR14224] antibody (ab187145)
All lanes : Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat kidney lysate
Lane 6 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 71 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PCK2 [EPR14224] antibody - C-terminal (ab187145)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1871454IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PCK2 [EPR14224] antibody - C-terminal (ab187145)
Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa cells, labeling PCK2 (green) with ab187145 at 1/500 dilution. Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
![Flow Cytometry - Anti-PCK2 [EPR14224] antibody (ab187145)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1871455FL.jpg)
Flow Cytometry - Anti-PCK2 [EPR14224] antibody (ab187145)
Flow cytometry analysis of PCK2 expression in 2% paraformaldehyde-fixed HeLa cells using ab187145 at 1/50 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 [EPR14224] antibody (ab187145)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab187145IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK2 [EPR14224] antibody (ab187145)
Immunohistochemical analysis of paraffin-embedded Human liver tissue, labeling PCK2 with ab187145 at 1/500 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
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