• Species Reactivity

  Mouse

• Specificity

  Detects mouse AMIGO3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant mouse AMIGO2 is observed and less than 2% cross-reactivity with recombinant human AMIGO1 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse AMIGO3    
  Thr20-Pro378    
  Accession # Q8C2S7

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse AMIGO3

• 
  

• Flow Cytometry

  2.5 µg/10    ⁶ cells

  Mouse splenocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMIGO3

AMIGO family proteins are cell adhesion molecules that exhibit homophilic and heterophilic binding properties and are thought to play roles in neuronal axon tract development and cell adhesion. Members of the AMIGO family of type I transmembrane proteins contain 6 leucine-rich repeats (LRRs) and one Ig domain in the extracellular region. Amphoterin-Induced Gene and ORF 3 (AMIGO3) is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (CRRs) and one Ig domain in their extracellular domains. It is ubiquitously expressed and is detected in all tissues studied. Mouse AMIGO3 shows less than 40% amino acid sequence homology with AMIGO1 and AMIGO2. The extracellular domain of mouse AMIGO3 shares 90% and 75% amino acid sequence identity with that of rat and human AMIGO3, respectively.

• Long Name:

  Amphoterin-induced Gene and ORF 3

• Entrez Gene IDs:

  386724 (Human); 320844 (Mouse)

• Alternate Names:

  adhesion molecule with Ig-like domain 3; ALI3; Alivin-3; AMIGO3; AMIGO-3; amphoterin-induced gene and ORF 3; amphoterin-induced protein 3; KIAA1851; MGC120552