• Species Reactivity

  Human

• Specificity

  Detects human Fibronectin in Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # P1H11

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human plasma-derived Fibronectin

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Human Fibronectin (Catalog # )

• 
  

• Simple Western

  20 µg/mL

  See below

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

• Immunoprecipitation

  Yu, H.     et al. (2004) Mol. Carcinog.     41:85.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibronectin

Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.

• Entrez Gene IDs:

  2335 (Human); 280794 (Bovine)

• Alternate Names:

  Fibronectin; FN1