Human Integrin beta 1/CD29 MAb (Clone P5D2) 25 UG

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Human Integrin beta 1/CD29 MAb (Clone P5D2) 25 UG信息二维码

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产品介绍

    基本参数

    详细说明

    • Species Reactivity

      Human

    • Specificity

      Detects human Integrin beta 1/CD29.

    • Source

      Monoclonal Mouse IgG    1 Clone # P5D2

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      Human skin keratinocytes

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

    • Endotoxin Level

      <0.10 EU per 1 μg of the antibody by the LAL method.  

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Flow Cytometry

      0.25 µg/10    6 cells

      See below


    • Immunoprecipitation

      Lin, Q. et al. (2004) Biochim. Biophys. Acta 1689:175.
      Joneckis, C.C. et al. (1993) Blood 82:3548.


    • Blockade of Receptor-ligand Interaction

      Mukhopadhyay, N.K.     et al. (2004) Ann. Thorac. Surg.     78:450.    
      Blaschke, F.     et al. (2002) Biochem. Biophys. Res. Commun.     296:890.


    • CyTOF-ready

      Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.


    • Immunocytochemistry

      8-25 µg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Flow Cytometry      
         

    Detection of Integrin  beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin  beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # , open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ).

    Immunocytochemistry      
         

    Integrin  beta 1/CD29 in human PBMCs. Integrin  beta 1/CD29 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Integrin  beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for .

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.5 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: Integrin beta 1/CD29

    The Integrin beta 1 subunit, also known as CD29, associates with at least ten different Integrin alpha subunits. It regulates not only its multiple ligands, but activates a signaling cascade in its expressing cells. CD29 is proposed to play a role in cell adhesion, apoptosis, and differentiation (1,2). Over amino acids (aa) 21-728, human and mouse share 92% aa sequence identity.

    • References:

      1. Barkan, D. and A.F. Chambers (2011) Clin. Cancer Res. 17:7219.

      2. Humphries, M.J. (2000) Biochem. Soc. Trans. 28:311.

    • Entrez Gene IDs:

      3688 (Human); 16412 (Mouse)

    • Alternate Names:

      CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA




















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