• Species Reactivity

  Human, Mouse, Rat, Chicken

• Specificity

  Detects human, mouse, rat, and chicken Pax7 (3-8).

• Source

  Monoclonal Mouse IgG    ₁ Clone # PAX7

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant chicken Pax7    
  aa 352-523

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pax7

Pax7 belongs to the paired box protein family of transcription factors. It is an important regulator of neural and skeletal muscle development (1 - 3).

• References:

1. Jostes, B. et al. (1990) Mech. Dev. 33(1):27.

2. Asakura, A. et al. (2001) Differentiation 68(4-5):245.

3. Kawakami, A. et al. (1997) Mech. Dev. 66:119.

4. Ericson, J. et al. (1996) Cell 87:661.

5. Berggren, K. et al. (2001) Dev. Dyn. 222:1.

6. Takebayashi, H. et al. (2002) Mech. Dev. 113:169.

7. McLoon, L.K. and J. Wirtschafter (2003) Invest. Opthamal. Vis. Sci. 44:1927.

8. Yamamoto, S. et al. (2001) J. Neurosci. 21:9814.

• Long Name:

  Paired Box Gene 7

• Entrez Gene IDs:

  5081 (Human); 18509 (Mouse); 500574 (Rat)

• Alternate Names:

  FLJ37460; HUP1; paired box 7; paired box gene 7; paired box homeotic gene 7; paired box protein Pax-7; paired domain gene 7; PAX7 transcriptional factor; Pax7; PAX7B; RMS2