• Species Reactivity

  Human

• Specificity

  Detects human Carbohydrate Sulfotransferase 3/CHST3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CHST1 or recombinant mouse CHST3 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 799011

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human Carbohydrate Sulfotransferase 3/CHST3    
  Glu39-Thr479    
  Accession # Q7LGC8

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbohydrate Sulfotransferase 3/CHST3

The human CHST family is comprised of 14 enzymes. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST3, also known as chondroitin 6-O-sulfotransferase, transfers sulfate to position 6 of GalNAc residues on chondroitin sulfate (3). Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Loss of CHST3 function in human results in severe chondrodysplasia (4). CHST3 can also sulfate Gal residues of keratan sulfate and Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides (5). The enzymatic activity of the recombinant human CHST3 was measured using a phosphatase-coupled sulfotransferease assay (6).

• References:

1. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.

2. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.

3. Uchimura, K. et al. (2002) J. Biol. Chem. 277:1443.

4. Thiele, H. et al. (2004) Proc. Natl. Acad. Sci. U. S. A. 101:10155.

5. Yusa, A. et al. (2006) J. Biol. Chem. 281: 20393.

6. Prather, B. et al. (2012) Anal. Biochem. 423:86.

• Entrez Gene IDs:

  9469 (Human); 53374 (Mouse); 84468 (Rat)

• Alternate Names:

  C6ST1; C6ST-1; C6ST1HSD; C6STEC 2.8.2.17; carbohydrate (chondroitin 6) sulfotransferase 3; Carbohydrate Sulfotransferase 3; Chondroitin 6-O-sulfotransferase 1; Chondroitin 6-sulfotransferase; CHST3; EC 2.8.2; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0; GST-0