• Species Reactivity

  Human, Rat

• Specificity

  Detect human and rat Neurogenin‑2.

• Source

  Monoclonal Mouse IgG    _2A Clone # 7G4

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant rat Neurogenin-2

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 µg/mL

  Immersion fixed paraffin-embedded sections of human brain

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurogenin-2

Neurogenin-2 (NgN2) is a member of neuroD-related bHLH transcription factor family. It is required for neurogenesis and can be used as a marker for cells of neuronal lineage.

• Entrez Gene IDs:

  63973 (Human); 11924 (Mouse); 295475 (Rat)

• Alternate Names:

  Atoh4; BHLHA8; bHLHa8MGC46562; Class A basic helix-loop-helix protein 8; Math4A; NEUROG2; neurogenin 2; Neurogenin2; Neurogenin-2; NGN2; ngn-2; Protein atonal homolog 4