• Species Reactivity

  Human

• Specificity

  Detects human CX3CL1/Fractalkine Chemokine Domain in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 81513

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 and     E. coli-derived recombinant human CX3CL1/Fractalkine Chemokine Domain    
  Gln25-Arg339 (Ser199Asn)    
  Accession # P78423

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human CX3CL1/Fractalkine Full Length (Catalog # )

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• Immunohistochemistry

  8-25 µg/mL

  Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CX3CL1/Fractalkine

CX3CL1 is a type I membrane protein in which an extracellular chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

• Entrez Gene IDs:

  6376 (Human); 20312 (Mouse); 89808 (Rat)

• Alternate Names:

  ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1