• Species Reactivity

  Mouse

• Specificity

  Detects mouse CCDC134 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human CCDC134 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 815606

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse CCDC134    
  Ala23-Leu229    
  Accession # Q8C7V8

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCDC134

Coiled-coil domain containing 134 (CCDC134) is a widely expressed glycoprotein that is secreted as a 38 kDa molecule. CCDC134 overexpression inhibits kinase activity in the Raf-1/MEK/ERK and JNK/SAPK pathways. In humans, CCDC134 polymorphisms are associated with reduced levels of Amyloid beta peptide (1-42) in the cerebrospinal fluid, a potential biomarker for Alzheimer’s disease. Alternative splicing of mouse CCDC134 generates a short isoform that lacks the N-terminal 37 amino acids including the signal peptide. Mouse CCDC134 shares 90% and 95% aa sequence identity with human and rat CCDC134, respectively.

• Long Name:

  Coiled-coil Domain Containing 134

• Entrez Gene IDs:

  79879 (Human); 76457 (Mouse); 500909 (Rat)

• Alternate Names:

  CCDC134; coiled-coil domain containing 134; coiled-coil domain-containing protein 134; dJ821D11.3; FLJ22349; MGC21013