• Species Reactivity

  Human

• Specificity

  Detects human RB1 in Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 607121

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E.coli-derived recombinant human RB1    
  Lys240-Asn406    
  Accession # P06400

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  See below

• 
  

• Simple Western

  1 µg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RB1

Retinoblastoma 1 protein (RB-1; also retinoblastoma-associated protein, pp110, and p105-Rb) is a 110 kDa tumor suppressor gene and member of the retinoblastoma protein family.  Human RB-1 is 928 amino acids in length.  The protein contains a Pocket domain (aa 373-771), which is comprised of two other domains, domain A (aa 373-573) and domain B (aa 640-771), and a “spacer” (aa 580-639).  The Pocket domain binds to threonine-phosphorylated domain C (aa 771-928), which thereby prevents interaction with heterodimeric E2F/DP transcription factor complexes.  Human RB-1 is 90% aa identical to mouse RB-1.  RB-1 is expressed in the retina.  The underphosphorylated, active form of RB-1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest.  Defects in RB-1 lead to the childhood cancer retinoblastoma.  

• Long Name:

  Retinoblastoma-associated protein

• Entrez Gene IDs:

  5925 (Human)

• Alternate Names:

  OSRC; osteosarcoma; p105-Rb; pp110; pRb; RB; RB1; retinoblastoma 1; retinoblastoma suspectibility protein; retinoblastoma-associated protein