• Species Reactivity

  Human

• Specificity

  Detects human CCRL2/CRAM‑A/B. Stains human CCRL2-transfected cells but not irrelevant transfectants. Detects both the CRAM-A and CRAM-B isoforms.

• Source

  Monoclonal Mouse IgG    _2B Clone # 152254

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human CRAM-A    
  Met1-Val356 (Val180Met)    
  Accession # NP_001124382

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  2.5 µg/10    ⁶ cells

  HL‑60 human acute promyelocytic leukemia cell line

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCRL2/CRAM-A/B

CCRL2, also known as CRAM-A, CRAM-B, CKRX, and HCR, is a seven-transmembrane G-protein linked receptor that shares homology with other human chemokine receptors. The isoforms CRAM-A and CRAM-B differ at their N-termini by the inclusion of an additional 12 amino acids on CRAM-A. CCRL2/CRAM‑A/B is expressed at varying levels on a variety of peripheral blood cells including monocytes, neutrophils, and T cells (1, 2).

• References:

1. Fan, P. et al. (1998) Biochem. Biophys. Res. Commun. 243:264.

2. Migeotte, I. et al. (2002) Eur. J. Immunol. 32:494.

• Long Name:

  CC Chemokine Receptor-like 2

• Entrez Gene IDs:

  9034 (Human)

• Alternate Names:

  ACKR5; C-C chemokine receptor-like 2; CCR11; CCR6; CCRL2; CCRL2/CRAM-A/B; chemokine (C-C motif) receptor-like 2; Chemokine receptor CCR11; Chemokine receptor X; CKRX; CKRXMGC116710; CRAM; CRAMA; CRAM-A; CRAMB; CRAM-B; FLJ55815; HCR; HCRMGC34104; Putative MCP-1 chemokine receptor