详细说明
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Species Reactivity
Mouse
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Specificity
Detects mouse Desert Hedgehog/Dhh N-Terminus in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse (rm) Shh N-terminal peptide (aa 25‑198) is observed. Additionally, in direct ELISAs, no cross‑reactivity with rmDhh C-terminal peptide (aa 199‑396) and rmIhh C-terminal peptide (aa 241‑449) is observed.
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Source
Polyclonal Goat IgG
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Purification
Antigen Affinity-purified
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Immunogen
E. coli-derived recombinant mouse Desert Hedgehog/Dhh
aa 23-198
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
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Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
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Label
Unconjugated
Applications
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Western Blot
0.1 µg/mL
Recombinant Mouse Desert Hedgehog (C23II), N-Terminus (Catalog # )
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Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse spinal cord (dorsal roots)
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
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Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
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Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Desert Hedgehog/Dhh
Dhh regulates testis development and spermatogenesis via interaction of its N-terminal domain with the receptors Patched and Patched-2. Hedgehog proteins are secreted as precursor molecules that undergo autocatalytic internal cleavage to yield an N-terminal domain that has signaling activity and a C-terminal domain that is active in precursor processing.