• Species Reactivity

  Mouse

• Specificity

  Detects mouse LYVE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD44 or recombinant human LYVE-1 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 223322

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  BaF/3 mouse pro-B cell line transfected with mouse LYVE-1    
  Ala24-Thr234    
  Accession # Q8BHC0

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

• Blockade of Receptor-ligand Interaction

  In a functional ELISA, 0.02-0.1 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of biotinylated Hyaluronan to immobilized Recombinant Mouse LYVE-1 (Catalog # ) coated at 5 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding.

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Immunocytochemistry

  8-25 µg/mL

  Immersion fixed LYVE-1 transfected cells

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LYVE-1

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1-3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9-11).

• References:

1. Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.

2. Evered, D. and J. Whelan (1989) Ciba Found. Symp. 143:1.

3. Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.

4. Banerji, S. et al. (1999) J. Cell Biol. 144:789.

5. Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.

6. Carreira, C.M. et al. (2001) Cancer Research 61:8079.

7. Jackson, D.J. et al. (2001) Trends Immunol. 22:317.

8. Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.

9. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.

10. Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.

11. Wiggle, J.T. and G. Oliver (1999) Cell 98:769.

• Long Name:

  Lymphatic Vessel Endothelial Hyaluronan Receptor 1

• Entrez Gene IDs:

  10894 (Human); 114332 (Mouse); 293186 (Rat)

• Alternate Names:

  cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; LYVE-1XLKD1; XLKD1