• Species Reactivity

  Mouse

• Specificity

  Detects mouse Testican 3/SPOCK3 in ELISAs. In sandwich immunoassays, less than 0.25% cross-reactivity with recombinant human (rh) Testican 1 is observed and no cross-reactivity with rhTestican 2 is observed.

• Source

  Monoclonal Rat IgG    ₁ Clone # 330403

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Testican 3/SPOCK3    
  Val26-Ile436    
  Accession # Q8BKV0

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Mouse Testican 3/SPOCK Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Mouse Testican 3/SPOCK3 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Mouse Testican 3/SPOCK3 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Mouse Testican 3/SPOCK3 Protein, CF (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Testican 3/SPOCK3

Testican 3 encoded by the SPOCK3 gene is a proteoglycan expressed in brain (1). Human and mouse Testican 3 share 90% amino acid sequence identity, indicating a conserved function (2, 3). Testican 3 contains Ca²⁺-binding domain and the C-terminal acidic domain with putative glycosaminoglycan attachment sites. In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. The N-terminal region, which is unique to Testicans, is responsible for the inhibition of Testican 3 towards MMP-14/MT1-MMP activation of MMP-2 (1). The thyropin domain and the follistatin-like domain with a six cysteine Kazal-like motif may inhibit cysteine and serine proteases, respectively (4). A spliced variant designated as N-Tes contains the N-terminal unique region, the follistatin-like domain and the Ca²⁺-binding domain, but lacks the C-terminal thyropin domain and the acidic domain (1).

• References:

1. Nakada, M. et al. (2001) Cancer Res. 61:8896.

2. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.

3. Okazaki, Y. et al. (2002) Nature 420:563.

4. Alliel, P.M. et al. (1993) Eur. J. Biochem. 214:347.

• Entrez Gene IDs:

  50859 (Human); 72902 (Mouse)

• Alternate Names:

  CWCV, and Kazal-like domains proteoglycan 3; HSAJ1454; sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3; SPOCK3; TES-3; Testican 3; TICN3