• Species Reactivity

  Mouse

• Specificity

  Detects mouse CD200 R1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD200 R1 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 330502

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse CD200 R1    
  Thr26-Pro238    
  Accession # BAE42266

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Mouse CD200 R1 Fc Chimera (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD200 R1

CD200 R1, also known as OX-2 receptor, is a 90 kDa, type I transmembrane protein that belongs to the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity (1‑3). The mouse CD200 R1 cDNA encodes a 326 aa precursor that includes a 25 aa signal sequence, a 213 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, mouse CD200 R1 shares 56% and 70% aa sequence identity with human and rat CD200 R1, respectively. The ECD of mouse CD200 R1 shares 69%, 38%, 79%, and 83% aa sequence identity with the ECD of CD200 R2, 3, 4, and a CD200 R-like molecule, respectively. CD200 R1 is expressed primarily on mast cells, basophils, macrophages, and dendritic cells, (5‑7) while its ligand, CD200, is widely distributed (8). Disruption of this receptor-ligand pair by knockout of the CD200 gene leads to increased macrophage number and activation, plus a predisposition to autoimmune disorders (9). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (10). The CD200 R-like molecules may interact differently with CD200 (11, 12). The cytoplasmic domain of CD200 R1 contains two non-ITIM tyrosine residues which are required for propagating its inhibitory signals (13‑15). CD200 R-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12 (4, 16).

• References:

1. Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165.

2. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483. 

3. Barclay, A.N. et al. (2002) Trends Immunol. 23:285. 

4. Wright, G.J. et al. (2003) J. Immunol. 171:3034. 

5. Shiratori, I. et al. (2005) J. Immunol. 175:4441. 

6. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348. 

7. Fallarino, F. et al. (2004) J. Immunol. 173:3748. 

8. Wright, G.J. et al. (2001) Immunology 102:173. 

9. Hoek, R.M. et al. (2000) Science 290:1768.

10. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.

11. Hatherley, D. et al. (2005) J. Immunol. 175:2469.

12. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.

13. Zhang, S. and J.H. Phillips (2006) J. Leukoc. Biol. 79:363.

14. Zhang, S. et al. (2004) J. Immunol. 173:6786.

15. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.

16. Voehringer, D. et al. (2004) J. Biol. Chem. 279:54117.

• Long Name:

  CD200 Receptor 1

• Entrez Gene IDs:

  131450 (Human); 57781 (Mouse); 102139962 (Cynomolgus Monkey)

• Alternate Names:

  CD200 R1; CD200 receptor 1; CD200R1; CD200RMOX2Rcell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200; CRTR2; HCRTR2; MOX2 receptor; MOX2R; OX2RCD200 cell surface glycoprotein receptor