• Species Reactivity

  Human

• Specificity

  Detects human STAT2 in direct ELISAs and Western blots.

• Source

  Monoclonal Rat IgG    _2A Clone # 967425

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human STAT2    
  Gln679-Phe851    
  Accession # P52630

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Simple Western

  10 µg/mL

  See below

• 
  

• Knockout Validated

  STAT2 is specifically detected in HeLa human cervical epithelialcarcinoma parental cell line but is not detectable in STAT2knockout HeLa cell line.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT2

Signal transducer and activator of transcription 2 (STAT2) is a member of the transcription factor STAT family with an apparent M.W. of 113 kDa in SDS-PAGE gels. Human STAT2 is 851 amino acids (aa) in length and contains one SH2 domain (aa 572-667). Splicing variants produce two isoforms for human STAT2. The short isoform has a 32 aa substitution and a 199 aa deletion corresponding to aa 621‑652 and aa 653‑851 in the long isoform, respectively. Human STAT2 shares 73% and 65% aa sequence identity with rat and mouse STAT2, in that order. STAT2 functions as a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta ), and it is also required for myogenic differentiation.

• Long Name:

  Signal Transducer and Activator of Transcription 2

• Entrez Gene IDs:

  6773 (Human); 20847 (Mouse)

• Alternate Names:

  interferon alpha induced transcriptional activator; ISGF-3; MGC59816; P113; signal transducer and activator of transcription 2; signal transducer and activator of transcription 2, 113kD; signal transducer and activator of transcription 2, 113kDa; STAT113; STAT2