详细说明
Species Reactivity
Human
Specificity
Detects human PSG-6 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG 1 Clone # 935926
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived transfected with human PSG-6
Met1-Gly424
Accession # Q00889Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
5 µg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Human PSG-6 by Western Blot. Western blot shows human serum from a pregnant donor (+) compared to a non-pregnant donor (-). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PSG-6 Monoclonal Antibody (Catalog # MAB8598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for PSG-6 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Immunohistochemistry | PSG-6 in Human Placenta. PSG-6 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human PSG-6 Monoclonal Antibody (Catalog # MAB8598) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # ). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for . |
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PSG-6
Pregnancy-specific beta-1-glycoprotein 6 is encoded by the PSG6 gene. High levels of PSG biosynthesis are restricted to the placenta syncytiotrophoblast and are essential for the maintenance of normal gestation in mammalian species.
Long Name:
Pregnancy-specific beta-1-glycoprotein 6
Entrez Gene IDs:
5675 (Human)
Alternate Names:
CGM3; pregnancy specific beta-1-glycoprotein 6; Pregnancy-specific beta-1-glycoprotein 10; Pregnancy-specific beta-1-glycoprotein 12; pregnancy-specific beta-1-glycoprotein 6; Pregnancy-specific glycoprotein 10; Pregnancy-specific glycoprotein 12; Pregnancy-specific glycoprotein 6; PS-beta-G-10; PS-beta-G-12; PS-beta-G-6; PSBG-10; PSBG-12; PSBG-6; PSG10; PSG12; PSG6; PSG-6; PSGGB