Human PSG-6 MAb (Clone 935926) 25 UG

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Human PSG-6 MAb (Clone 935926) 25 UG信息二维码

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产品介绍

    基本参数

    详细说明

    • Species Reactivity

      Human

    • Specificity

      Detects human PSG-6 in direct ELISAs and Western blots.    
       

    • Source

      Monoclonal Mouse IgG    1 Clone # 935926

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      Human embryonic kidney cell line HEK293-derived transfected with human PSG-6    
      Met1-Gly424    
      Accession # Q00889

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      0.5 µg/mL

      See below


    • Immunohistochemistry

      5 µg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Human PSG-6 by Western Blot. Western blot shows human serum from a pregnant donor (+) compared to a non-pregnant donor (-). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PSG-6 Monoclonal Antibody (Catalog # MAB8598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for PSG-6 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Immunohistochemistry      
         

    PSG-6 in Human Placenta. PSG-6 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human PSG-6 Monoclonal Antibody (Catalog # MAB8598) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # ). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for .

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.5 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: PSG-6

    Pregnancy-specific beta-1-glycoprotein 6 is encoded by the PSG6 gene. High levels of PSG biosynthesis are restricted to the placenta syncytiotrophoblast and are essential for the maintenance of normal gestation in mammalian species.

    • Long Name:

      Pregnancy-specific beta-1-glycoprotein 6

    • Entrez Gene IDs:

      5675 (Human)

    • Alternate Names:

      CGM3; pregnancy specific beta-1-glycoprotein 6; Pregnancy-specific beta-1-glycoprotein 10; Pregnancy-specific beta-1-glycoprotein 12; pregnancy-specific beta-1-glycoprotein 6; Pregnancy-specific glycoprotein 10; Pregnancy-specific glycoprotein 12; Pregnancy-specific glycoprotein 6; PS-beta-G-10; PS-beta-G-12; PS-beta-G-6; PSBG-10; PSBG-12; PSBG-6; PSG10; PSG12; PSG6; PSG-6; PSGGB



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