• Species Reactivity

  Human

• Specificity

  Detects human  alpha -Smooth Muscle Actin.

• Source

  Monoclonal Mouse IgG    _2A Clone # 1A4

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 594

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: alpha-Smooth Muscle Actin

alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

• References:

1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.

2. Oishi, K. et al. (2002) J. Physiol. 540:139.

• Long Name:

  Actin, Alpha 2, Smooth Muscle, Aorta

• Entrez Gene IDs:

  59 (Human); 11475 (Mouse); 81633 (Rat)

• Alternate Names:

  AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin