• Species Reactivity

  Mouse

• Specificity

  Detects human Aminoacylase/ACY1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human ACY1 is observed.

• Source

  Monoclonal Rat IgG    _2B Clone # 475730

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Aminoacylase/ACY1    
  Met1-Ser408    
  Accession # NP_079647

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Mouse Aminoacylase/ACY1 (Catalog # )

• 
  

• Immunoprecipitation

  25 µg/mL

  Cell lysates spiked with Recombinant Mouse Aminoacylase/ACY1 (Catalog # ),

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminoacylase/ACY1

Aminoacylase 1 (ACY1) is an intracellular soluble homodimeric zinc-binding enzyme. It is involved in the breakdown of acetylated amino acids generated during protein degradation by catalyzing the formation of free aliphatic amino acids from N-acetylated precursors. This enzyme is evolutionarily conserved between mouse and human. Mutations in the human ACY1 gene might cause inborn metabolic disorders. The amino acid sequence of mouse ACY1 is 93% and 85% identical to that of rat and human/chimpanzee.

• Entrez Gene IDs:

  95 (Human); 109652 (Mouse)

• Alternate Names:

  ACY1; ACY-1; ACY1D; acylase; aminoacylase 1; Aminoacylase; aminoacylase-1; EC 3.5.1; EC 3.5.1.14; N-acyl-L-amino-acid amidohydrolase