• Species Reactivity

  Human

• Specificity

  Detects human Histamine H1 R. Stains human Histamine H1 R transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 480054

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human Histamine H1 R    
  Met1-Ser487    
  Accession # AAH60802

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  2.5 µg/10    ⁶ cells

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Histamine H1 R

Histamine H1 R (HRH1) is a 60 kDa, 487 aa G-protein coupled 7-transmembrane putative glycoprotein. It is implicated in the pathogenesis of asthma and is a major pharmaceutical target for antihistamines. It has been detected as a mixture of monomers and homodimers, with highest concentration in the placenta, followed by brain, lung (produced by airway smooth muscle, bronchial epithelium and macrophages) and other tissues. Extracellular domains of human HRH1 share 72% aa sequence identity with corresponding regions of mouse or rat HRH1.

• Long Name:

  Histamine H1 Receptor

• Entrez Gene IDs:

  3269 (Human); 15465 (Mouse); 24448 (Rat)

• Alternate Names:

  H1R; H1-R; hisH1; Histamine H1 R; Histamine H1R; histamine receptor H1; HRH1; subclass H1