• Species Reactivity

  Human

• Specificity

  Detects human LCoR in direct ELISAs.

• Source

  Monoclonal Mouse IgG    _2B Clone # 731229

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human LCoR    
  Asn110-Gln338    
  Accession # Q96JN0

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LCoR

LCoR (ligand-dependent corepressor), also known as MLR2 (Mblk1-related protein 2), is a 47-50 kDa nuclear phosphoprotein that is a corepressor of ligand-dependent transcriptional activation by nuclear receptors such as estrogen and progesterone receptors. It is widely expressed throughout fetal development and in the adult. The 433 amino acid (aa) LCoR contains two N-terminal PxDLS nuclear receptor interaction motifs, a central HDAC interaction motif, a nuclear localization signal, and a C‑terminal Helix-Turn-Helix motif. Alternate splicing produces a truncated 406 aa variant. Within the region used as an immunogen, human LCoR shares 97% aa identity with mouse and rat LCoR.

• Long Name:

  Ligand-dependent Nuclear Receptor Corepressor

• Entrez Gene IDs:

  84458 (Human); 212391 (Mouse); 365462 (Rat)

• Alternate Names:

  FLJ38026; KIAA1795RP11-175O19.1; LCoR; ligand dependent nuclear receptor corepressor; Mblk1-related protein 2; MLR2; MLR2ligand-dependent corepressor