• Species Reactivity

  Rat

• Specificity

  Detects rat Serpin A12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human Serpin A12 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant rat Serpin A12    
  Leu20-Pro411    
  Accession # Q8R4Z1

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Rat Serpin A12

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Rat Serpin A12,

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A12

Serpin A12 (serine proteinase inhibitor-clade A #12; also vaspin) is a 45‑50 kDa, secreted member of the alpha 1-antitrypsin (clade A)-subfamily, Serpin superfamily of protease inhibitors. It is produced by visceral and subcutaneous adipocytes, plus adipocyte-derived stem cells during obesity or diabetes. The presence of vaspin reverses insulin-resistance. Its molecular target is unknown, but it may antagonize the action of proteases that impair the action of insulin. Rat Serpin A12 is 411 amino acids (aa) in length. It contains a 20 aa signal sequence and a 391 aa mature region. A potential “active” inhibitor site lies between Gly364 and Pro389. Mature rat Serpin A12 shares 62% and 88% aa identity with human and mouse Serpin A12, respectively.

• Entrez Gene IDs:

  145264 (Human); 68054 (Mouse); 191570 (Rat)

• Alternate Names:

  antitrypsin), member 12; member 12; OL-64; OL-64Visceral adipose-specific serpin; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A12; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); Vaspin; Visceral adipose tissue-derived serine protease inhibitor