• Species Reactivity

  Rat

• Specificity

  Detects rat CX3CL1/Fractalkine in ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Eotaxin is observed. Neutralizes the biological activity of rhCX3CL1 and rmCX3CL1 with similar effectiveness.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant rat CX3CL1/Fractalkine    
  Gln25-Gly100    
  Accession # O55145

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Rat CX3CL1/Fractalkine (Catalog # )

• 
  

• Immunohistochemistry

  5-15 µg/mL

  See below

• 
  

• Rat CX3CL1/Fractalkine Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  0.2-0.8 µg/mL 

  Rat CX3CL1/Fractalkine Chemokine Domain Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Rat CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Rat CX3CL1/Fractalkine Protein (Catalog # )

• 
  

• Neutralization

  Measured by its ability to neutralize CX3CL1/Fractalkine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CX3CR1. The Neutralization Dose (ND     ₅₀) is typically 0.3-1.2 µg/mL in the presence of 40 ng/mL Recombinant Rat CX3CL1/Fractalkine.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CX3CL1/Fractalkine

CX3CL1, also named neurotactin, is a member of the delta chemokine subfamily that contains a novel C-X₃-C motif. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Rat CX3CL1 cDNA encodes a 393 amino acid (aa) residue precursor protein with two alternative (21 aa or 24 aa residue) putative signal peptides, a 74 aa or 76 aa residue globular chemokine domain, a 238 aa residue stalk region rich in Gly, Pro, Ser and Thr and containing degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 36 aa residue cytoplasmic domain. The extracellular domain of CX3CL1 can potentially be released as a soluble protein by proteolysis at the conserved dibasic motif proximal to the transmembrane region. With the exception of the stalk region, rat CX3CL1 shares a high degree of amino acid sequence homology (83% sequence identity) with human and mouse CX3CL1. CX3CL1 is expressed in various tissues including heart, brain, lung, kidney, skeletal muscle, and testis. In rat brain, CX3CL1 expression was found to be localized principally to neurons. The expression of CX3CL1 was also reported to be up-regulated on activated endothelial cells. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T‑lymphocytes but not monocytes. CX3CR1, previously named V28 or chemokine beta receptor‑like 1, has been found to be a specific receptor for CX3CL1. In addition, US28, a 7TM receptor encoded by human cytomegalovirus that binds multiple CC chemokines, has also been shown to bind CX3CL1 with high-affinity.

• References:

1. Kledal, T.N. et al. (1998) FEBS Lett. 441:209.

2. Combadiere, C. et al. (1998) J. Biol. Chem. 273:23799.

3. Harrison, J.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10896.

4. Rossi, D.L. et al. (1998) Genomics 47:163.

• Entrez Gene IDs:

  6376 (Human); 20312 (Mouse); 89808 (Rat)

• Alternate Names:

  ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1