• Species Reactivity

  Equine

• Specificity

  Detects equine IL-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross‑reactivity with recombinant human IL-2, recombinant mouse IL-2, recombinant rat IL-2, recombinant feline IL-2, recombinant bovine IL-2, recombinant canine IL-2, recombinant cotton rat IL-2, and recombinant porcine IL-2 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant equine IL‑2    
  Ala21-Thr149 (Cys141Ser)    
  Accession # NP_001078902.1

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Equine IL‑2 (Cys141Ser) (Catalog # )

• 
  

• Immunocytochemistry

  5-15 µg/mL

  See below

• 
  

• Equine IL-2 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  0.2-0.8 µg/mL 

  Equine IL‑2 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 Not Assigned 

  Equine IL‑2 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Equine IL-2 (Cys141Ser) Protein (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens (1). IL-2 has since been found to also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes (2).

The biological activity of IL-2 is mediated by the binding of IL-2 to cell surface receptor complexes. The functional high-affinity receptor that mediate IL-2 signals is composed of three polypeptide chains, the IL-2 receptor alpha, beta and gamma subunits (3). IL-2 also signals via the intermediate affinity receptor complex of the beta  and gamma subunits (4). In T cells, the beta  and gamma subunits are shared with the IL-15 receptor complex (5). The gamma subunit of the IL-2 receptor complex has also been shown to be a subunit of the receptor complexes of IL-4, IL-7, IL-9 and IL-21 (6).

At the amino acid sequence level, equine IL-2 shares 72%, 70%, 56% and 54% sequence similarities with human, porcine, rat and mouse IL-2, respectively. It has been reported that equine IL-2 augmented proliferation in equine peripheral blood mononuclear cells, but has no effect on mouse CTLL-2 cells (7).

• References:

1. Morgan, D.A. et al. (1976) Science 193:1007.

2. Smith, K.A. et al. (1988) Science 240:1169.

3. Taniguchi, T. and Y. Minami (1993) Cell 73:5.

4. Giri, J. et al. (1994) EMBO J. 13:2822.

5. Waldmann, T. et al. (1998) Int. Rev. Immunol. 16:205.

6. Nelson, B.H. and D.M. Willeford (1998) Adv. Immunol. 70:1.

7. E.V. Vandergrift and D.W. Horohov (1993) Vet. Immunol. Immunopathol. 39:395.

• Long Name:

  Interleukin 2

• Entrez Gene IDs:

  3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

• Alternate Names:

  aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF