• Species Reactivity

  Canine

• Specificity

  Detects canine CCL2/JE/MCP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) CCL2/MCP-1 is observed, approximately 10% cross-reactivity with recombinant mouse (rm) JE, recombinant rat JE, and recombinant cotton rat JE is observed, and less than 5% cross-reactivity with rhEotaxin, rmMARC, and rhMCP-2 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant canine CCL2/JE/MCP-1    
  Gln24-Pro101    
  Accession # P52203

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Canine CCL2/JE/MCP‑1 (Catalog # )

• 
  

• Immunocytochemistry

  5-15 µg/mL

  See below

• 
  

• Neutralization

  Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND    ₅₀) is typically 0.8-4.0 µg/mL in the presence of 300 ng/mL Recombinant Canine CCL2/JE/MCP‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL2/JE/MCP-1

Canine MCP-1 (monocyte chemotactic protein-1) is an 8 kDa member of the CC chemokine family of chemotactic factors (1, 2). It is synthesized as a 101 amino acid (aa) precursor that contains a 23 aa signal sequence and a 78 aa mature segment (3). It contains no potential N-linked glycosylation sites and is not known for any posttranslational modifications. Based on human studies, MCP-1 will primarily circulate as a monomer. Noncovalent dimers are likely to be found, however.
MCP‑1 activity has been localized to the N-terminus (1). Cell types known to secrete MCP-1 are considerable in number, and include keratinocytes, fibroblasts, endothelium, osteoblasts, macrophages, mast cells, smooth muscle cells and astrocytes (1, 2). In the mature MCP-1 segment, there is 82% and 83% aa identity, canine to human and porcine MCP-1, respectively. When mature canine MCP-1 is compared to (125 aa) extended rodent MCP-1, there is 55% and 56% aa identity, canine to mouse and rat MCP-1, respectively. MCP-1 has three possible receptors. The first two are CCR2 (1) and CCR11 (4). The third receptor has only been identified in mice and is called L-CCR (5). Its function is unknown. MCP-1 is best known as a chemotactic agent for mononuclear cells. It also, however, induces enzyme and cytokine release in monocytes, NK cells, and lymphocytes and histamine release by basophils (1). Additionally, it is believed to reduce IL-12 production by dendritic cells and promote a Th2 phenotype in CD4⁺ T cells (6).

• References:

1. Coillie, E.V. et al. (1999) Cytokine Growth Factor Rev. 10:61.

2. Yoshie, O. et al. (2001) Adv. Immunol. 78:57.

3. Kumar, A.G. et al. (1997) Circulation 95:693.

4. Biber, K. et al. (2003) J. Leukoc. Biol. 74:243.

5. Luther, S.A. and J.G. Cyster  (2001) Nat. Immunol. 2:102.

• Entrez Gene IDs:

  6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

• Alternate Names:

  CCL2; MCAF; MCP-1