• Species Reactivity

  Feline

• Specificity

  Detects IL‑1 beta /IL‑1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human IL‑1 beta, recombinant mouse IL-1 beta, and recombinant canine IL-1 beta is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant feline IL‑1 beta /IL‑1F2    
  Ala116-Ser267    
  Accession # P41687

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Feline IL‑1 beta /IL‑1F2 (Catalog # )

• 
  

• Immunocytochemistry

  5-15 µg/mL

  Immersion fixed feline peripheral blood mononuclear cells

• 
  

• Feline IL-1 beta /IL-1F2 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  0.2-0.8 µg/mL 

  Feline IL‑1 beta /IL‑1F2 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Feline IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Feline IL-1 beta/IL-1F2 Protein (Catalog # )

• 
  

• Neutralization

  Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A.      et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J.      et al. (eds): IRL Press. 272. The Neutralization Dose (ND     ₅₀) is typically 0.02-0.08 µg/mL in the presence of 50 pg/mL Recombinant Feline IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in feline. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with the IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1‑4). The feline IL-1 beta cDNA encodes a 267 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature feline IL-1 beta shares 63%‑78% aa sequence identity with canine, cotton rat, equine, human, mouse, porcine, rat, and rhesus IL-1 beta.

• References:

1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.

2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.

3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.

4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.

5. Accession # AAA30814.

6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

• Long Name:

  Interleukin 1 beta

• Entrez Gene IDs:

  3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

• Alternate Names:

  catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta