• Species Reactivity

  Equine

• Specificity

  Detects equine IL-4 in ELISAs and Western blots. In sandwich ELISAs, less than 0.7% cross-reactivity with recombinant human IL‑4, recombinant mouse IL‑4, recombinant rat IL-4, recombinant cotton rat IL-4, recombinant canine IL-4, recombinant feline IL-4, recombinant porcine IL-4, recombinant bovine IL‑4, and recombinant rhesus macaque IL-4 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant equine IL‑4    
  Lys26-Cys137    
  Accession # NP_001075988

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Equine IL‑4 (Catalog # )

• 
  

• Immunocytochemistry

  5-15 µg/mL

  See below

• 
  

• Equine IL-4 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  0.2-0.8 µg/mL 

  Equine IL‑4 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Equine IL‑4 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Equine IL-4 Protein (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1-3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Equine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature equine IL-4 shares 53-60% aa sequence identity with bovine, goat, human, ovine, and porcine IL-4 and 38-40% aa sequence identity with mouse and rat IL-4. IL-4 exerts its effects through two receptor complexes (5, 6). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non-hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4⁺ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (7-10). IL-4 plays a dominant role in the development of allergic inflammation and asthma (9, 11).

• References:

1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.

2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.

3. Vandergrifft, E.V. et al. (1994) Vet. Immunol. Immunopathol. 40:379.

4. Redfield, C. et al. (1991) Biochemistry 30:11029.

5. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.

6. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.

7. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.

8. Corthay, A. (2006) Scand. J. Immunol. 64:93.

9. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.

10. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.

11. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

• Long Name:

  Interleukin 4

• Entrez Gene IDs:

  3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)

• Alternate Names:

  B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra