• Species Reactivity

  Human

• Specificity

  Detects human FGL2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Angiopoietin-1, rhAngiopoietin-2, rhAngiopoietin-like 2, and recombinant mouse Angiopoietin-3 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 532223

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human FGL2    
  Asn24-Pro439    
  Accession # Q14314

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Immunocytochemistry

  3-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGL2

FGL2 (fibrinogen-like protein 2), also called fibroleukin, is a 64‑70 kDa secreted glycoprotein of the fibrinogen-like superfamily. It is produced by macrophages and endothelial cells in response to inflammatory mediators, T cells and fetal trophoblast cells. FGL2 has prothrombinase activity, promotes immune-mediated thrombosis and functions in transplant rejection and infection-related abortion. Mature human FGL2 gene is a 416 amino acid (aa) protein with a coiled-coil region and a fibronectin C-terminal homology domain or FRED. Coiled-coil dimers are covalently linked to form a tetrameric 260‑280 aa FGL2 complex. Mature human FGL2 shares 79% aa identity with mouse FGL2.

• Long Name:

  Fibrinogen-like 2

• Entrez Gene IDs:

  10875 (Human); 14190 (Mouse); 84586 (Rat)

• Alternate Names:

  FGL2; fibrinogen-like 2; Fibrinogen-like protein 2; Fibroleukin; pT49T49; T49