• Species Reactivity

  Mouse

• Specificity

  Detects mouse TLR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-1 RI, II, 3, 6, 9, recombinant human IL-1 R8, rmIL-18 R, rmIL-18 Rb, rmMD-1, rmRP105, rmSIGIRR, rmTLR2, 3, 4, or 6 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 285923

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse TLR1    
  Ser25-Asp581    
  Accession # Q9EPQ1

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Mouse TLR1 (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR1

The Toll-like family of molecules are type I transmembrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least twelve mouse and ten human TLRs that activate the innate immune system following exposure to a variety of microbial species (1, 2). TLRs contain a large number of leucine-rich repeats (LRRs) and a cytoplasmic tail with one Toll/IL-1 receptor (TIR) domain. Mature mouse TLR1 consists of a 557 amino acid (aa) extracellular domain (ECD) with 20 LRRs, a 21 aa transmembrane segment, and a 192 aa cytoplasmic domain (3, 4). Within the ECD, mouse TLR1 shares 60% aa sequence identity with mouse TLR6 and 19%‑27% aa sequence identitity with mouse TLR2, -3, -4, -5, -7, -8, -9, -11, -12, and -13. It shares 73% and 86% aa sequence identity with human and rat TLR1, respectively. TLR1 is expressed on the surface of macrophages, dendritic cells, and tonsillar epithelial cells in ligand-independent association with TLR2 (5‑8). TLR2 additionally associates with TLR6 to form a functional complex with specificity for distinct but related microbial ligands (9‑11). TLR1 and TLR2 cooperate in the recognition of bacterial and protozoal triacylated lipopeptides and glycosylphosphatidylinositols (6, 10‑12). Ligand binding induces TLR1 localization to lipid rafts followed by receptor internalization and activation of NF kappa B (7, 11, 13).

• References:

   

1. Miyake, K. (2007) Semin. Immunol. 19:3.

2. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.

3. SwissProt # Q9EPQ1.

4. Matsushima, N. et al. (2007) BMC Genomics 8:124.

5. Ochoa, M.-T. et al. (2003) Immunology 108:10.

6. Takeuchi, O. et al. (2002) J. Immunol. 169:10.

7. Triantafilou, M. et al. (2006) J. Biol. Chem. 281:31002.

8. Sandor, F. et al. (2003) J. Cell Biol. 162:1099.

9. Nakao, Y. et al. (2005) J. Immunol. 174:1566.

10. Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. 97:13766.

11. Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.

12. Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.

13. Nishiya, T. and A.L. DeFranco (2004) J. Biol. Chem. 279:19008.

• Long Name:

  Toll-like Receptor 1

• Entrez Gene IDs:

  7096 (Human); 21897 (Mouse); 305354 (Rat)

• Alternate Names:

  CD281 antigen; CD281; DKFZp547I0610; DKFZp564I0682; KIAA0012MGC126311; MGC104956; MGC126312; rsc786; TIL; TIL. LPRS5; TLR1; Toll/interleukin-1 receptor-like protein; toll-like receptor 1