• Species Reactivity

  Mouse

• Specificity

  Detects mouse Angiopoietin-like 3 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Angoipoietin-1, rhAngoipoietin-2, rhAngoipoietin-4, rmAngoipoietin-3, and cornea-derived transcript 6 (CDT6), an Angiopoietin-like factor.

• Source

  Monoclonal Rat IgG    _2A Clone # 128610

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant mouse Angiopoietin-like 3    
  Ser17-Thr455    
  Accession # Q9R182

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Mouse Angiopoietin-like 3 (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 3/ANGPTL3

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins (1-3). Mature mouse ANGPTL3 contains an N-terminal coiled-coil domain and a C‑terminal fibrinogen-like domain (4). ANGPTL3 is expressed in the liver from early in development through adulthood (4, 5). Full length ANGPTL3 circulates in the plasma as do the proteolytically separated N- and C-terminal fragments containing the coiled-coil domain and fibrinogen-like domains, respectively (6, 7). ANGPTL3 is found as 70 kDa, 50 kDa, and 32 kDa species and can form weakly associated noncovalent multimers   in vitro (5, 6). ANGPTL3 directly inhibits lipoprotein lipase (LPL), an enzyme responsible for hydrolyzing circulating triglycerides (8). This activity requires a putative heparin-binding motif that is N-terminal to the coiled-coil domain (6). Proteolytic removal of the fibrinogen-like domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL   in vitro and function   in vivo (6). ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake (6-8). ANGPTL3 knockout mice are hypolipidemic and have elevated LPL activity (9). ANGPTL3 expression   in vivo is upregulated by LXR agonists and downregulated by insulin, leptin, and TR beta agonists (10-12). Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice, (7, 8, 11). ANGPTL3 does not bind Tie-1 or Tie-2 but its fibrinogen-like domain interacts with integrin alpha V beta 3 to induce endothelial cell adhesion, migration, and neovascularization (13). ANGPTL3, secreted by fetal liver cells, also promotes the expansion of hematopoietic stem cells (14). Mature mouse ANGPTL3 shares 22%‑30% amino acid (aa) sequence identity with ANGPTL1, 2, 4, 6, and 7. It shares 77% aa sequence identity with human ANGPTL3.

• References:

1. Li, C. (2006) Curr. Opin. Lipidol. 17:152.

2. Oike, Y. et al. (2004) Int. J. Hematol. 80:21.

3. Kersten, S. (2005) Biochem. Soc. Transact. 33:1059.

4. Conklin, D. et al. (1999) Genomics 62:477.

5. Ge, H. et al. (2005) J. Lipid Res. 46:1484.

6. Ono, M. et al. (2003) J. Biol. Chem. 278:41804.

7. Koishi, R. et al. (2002) Nat. Genet. 30:151.

8. Shimizugawa, T. et al. (2002) J. Biol. Chem. 277:33742.

9. Koster, A. et al. (2005) Endocrinology 146:4943.

10. Inaba, T. et al. (2003) J. Biol. Chem. 278:21344.

11. Shimamura, M. et al. (2004) Biochem. Biophys. Res. Commun. 322:1080.

12. Fugier, C. et al. (2006) J. Biol. Chem. 281:11553.

13. Camenisch, G. et al. (2002) J. Biol. Chem. 277:17281.

14. Zhang, C.C. et al. (2006) Nat. Med. 12:240.

• Entrez Gene IDs:

  27329 (Human); 30924 (Mouse)

• Alternate Names:

  AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2