• Species Reactivity

  Human

• Specificity

  When used in combination with the biotinylated anti-FGF-4 detection antibody (Catalog # BAF235) in sandwich ELISAs, no significant cross-reactivity or interference was observed with recombinant human (rh) FGF acidic, bFGF acidic, rhFGF basic, bFGF basic, rhFGF-5, rhFGF-6, or rhFGF-7 (KGF).    
   

• Source

  Monoclonal Mouse IgG    ₁ Clone # 26019

• Purification

  Protein A or G purified from ascites

• Immunogen

  E. coli-derived recombinant human FGF-4    
  Accession # P08620

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Human FGF-4 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Human FGF‑4 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Human FGF‑4 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human FGF-4 Protein (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-4

FGF-4 is a member of the FGF family of growth factors. FGF-4 is important in embryonic angiogenesis and limb development and is mitogenic for fibroblasts and endothelial cells.

• Long Name:

  Fibroblast Growth Factor 4

• Entrez Gene IDs:

  2249 (Human); 14175 (Mouse); 116499 (Rat)

• Alternate Names:

  FGF4; FGF-4; fibroblast growth factor 4; HBGF-4; HBGF-4Transforming protein KS3; heparin secretory transforming protein 1; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; HST-1; HST-1HSTF-1; HSTF1fibroblast growth factor 4 splice isoform; HSTFGF-4; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; KFGF; K-FGF; KS3; oncogene HST