• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human, mouse, and rat GSK-3 beta  in ELISAs and Western blots. Does not cross-react with recombinant human GSK-3 alpha.

• Source

  Monoclonal Rat IgG    _2B Clone # 272536

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human GSK-3 beta    
  Met1-Thr420    
  Accession # P49841

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Intracellular Staining by Flow Cytometry

  2.5 µg/10    ⁶ cells

  HeLa human cervical epithelial carcinoma cell line fixed with paraformaldehyde and permeabilized with methanol.    
   

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GSK-3 beta

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.

• Long Name:

  Glycogen Synthase Kinase 3 beta

• Entrez Gene IDs:

  2932 (Human); 56637 (Mouse); 84027 (Rat)

• Alternate Names:

  EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK3 beta; GSK-3 beta; GSK3B; GSK3beta isoform