详细说明
Species Reactivity
Human
Specificity
Detects human IL-2 R beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R alpha, rh gamma c, rhIL‑4 R, or rhIL‑6 R is observed.
Source
Monoclonal Mouse IgG 1 Clone # 27302
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑2 R beta
Ala27-Asp239
Accession # NP_000869Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Flow Cytometry
2.5 µg/10 6 cells
Human whole blood CD56 + natural killer cells
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization
Measured by its ability to neutralize IL‑2-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND 50) is typically 0.05-0.15 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Neutralization | Cell Proliferation Induced by IL‑2 and Neutralization by Human IL‑2 R beta Antibody. Recombinant Human IL‑2 (Catalog # ) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑2 R beta Monoclonal Antibody (Catalog # MAB224). The ND50 is typically 0.05‑0.15 µg/mL. |
Immunohistochemistry | IL‑2 R beta in Human Lymph Node. IL‑2 R beta was detected in immersion fixed frozen sections of human lymph node using 15 µg/mL Mouse Anti-Human IL‑2 R beta Monoclonal Antibody (Catalog # MAB224) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for . |
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-2 R beta
Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains and the intermediate affinity complex comprising heterodimers of the beta and gamma chains. Individual beta chains and alpha chains exhibit low affinity IL-2 binding, and the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain have been shown to be required for IL-15 mediated signaling. IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid (aa) precursor Type I membrane protein with a 26 aa signal peptide, a 214 aa extracellular region, a 25 aa transmembrane region and a 286 aa cytoplasmic domain. A soluble IL-2 R beta has been identified in the culture supernatants of a human lymphoid cell line, YT, that displays IL-2 R beta. Soluble IL-2 R beta binds IL-2 with low affinity and is not an effective IL-2 antagonist on cells displaying the high or intermediate affinity IL-2 signaling receptors. Nevertheless, soluble IL-2 R beta binds IL-15 with sufficient affinity to neutralize IL-15 biological activities.
Long Name:
Interleukin 2 Receptor beta
Entrez Gene IDs:
3560 (Human); 16185 (Mouse)
Alternate Names:
CD122 antigen; CD122; high affinity IL-2 receptor beta subunit; High affinity IL-2 receptor subunit beta; IL-15 R beta; IL-2 R beta; IL-2 receptor subunit beta; IL2R beta; IL-2R subunit beta; IL2RB; IL-2Rb; interleukin 15 receptor, beta; interleukin 2 receptor, beta; interleukin-2 receptor subunit beta; P70-75; p75