• Species Reactivity

  Human

• Specificity

  Detects human C1q R1/CD93 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse C1q R1 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 273107

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human C1q R1/CD93    
  Ala24-Lys580    
  Accession # Q9NPY3

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human C1q R1/CD93 under non-reducing conditions only

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• Flow Cytometry

  2.5 µg/10    ⁶ cells

  Human whole blood monocytes

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• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C1q R1/CD93

C1q R1 is also known as C1q Rp, Collectin Receptor, and AA4 antigen, and is designated CD93. C1q R1 mediates enhanced phagocytosis by monocytes and macrophages upon interaction with soluble defense collagens including C1q, MBL, and SP-A (1, 2). It is a type I transmembrane glycoprotein expressed on endothelial cells, hematopoietic progenitor cells, platelets, and cells of myeloid origin. C1q R1 has also been identified as a stem cell marker (3, 4).

• References:

1. Kim, T.S. et al. (2000) Mol. Immunol. 37:377.

2. Dean, Y.D. et al. (2001) Eur. J. Immunol. 31:1370.

3. Petrenko, O. et al. (1999) Immunity 10:691.

4. Danet, G.H. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10441.

• Long Name:

  Complement Component C1q Receptor

• Entrez Gene IDs:

  22918 (Human); 17064 (Mouse); 84398 (Rat)

• Alternate Names:

  AA4 Antigen; C1q R1; C1q receptor 1; C1q Rp; C1q/MBL/SPA receptor; C1qR(P); C1qR1; C1qRp; CD93 antigenC1qR; CD93 molecule; CD93; CDw93C1QR1; Collectin Receptor; Complement component 1 q subcomponent receptor 1; complement component 1, q subcomponent, receptor 1; complement component C1q receptor; dJ737E23.1; ECSM3; Ly68; Matrix-remodeling-associated protein 4; matrix-remodelling associated 4; MXRA4