• Species Reactivity

  Human

• Specificity

  Detects human CD27 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) 4‑1BB, rhBAFF R, recombinant mouse (rm) CD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGF R, rhOPG, rmOX40, rhRANK, rhTAJ, or rhTNF RI is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 57703

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human CD27    
  Thr21-Ile192    
  Accession # P26842

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human CD27/TNFRSF7 Fc Chimera (Catalog # )

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• Flow Cytometry

  2.5 µg/10    ⁶ cells

  Human whole blood lymphocytes

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• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

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• Neutralization

  Measured by its ability to neutralize CD27/TNFRSF7-induced inhibition of proliferation in mouse splenic T cells. The Neutralization Dose (ND    ₅₀) is typically 0.2-0.4 µg/mL in the presence of 3 µg/mL Recombinant Human CD27/TNFRSF7 Fc Chimera, 10 µg/mL Recombinant Mouse CD27 Ligand/TNFSF7, and sub-optimal amounts of Mouse CD3 epsilon Monoclonal Antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD27/TNFRSF7

Human CD27 is a lymphocyte-specific member of the TNF receptor superfamily. CD27 is expressed on a subset of human thymocytes and on the majority of mature T cells. CD27 expression is up-regulated after TCR stimulation. Within the CD4⁺ compartment, it is preferentially expressed on CD45RA⁺ cells. In contrast, it is preferentially expressed on CD45RO⁺ cells in the CD8⁺ compartment. CD27 also appears to be a potential marker for memory B cells. It exists as both a disulfide‑linked dimer on the cell surface and as a soluble protein found in serum. Human CD27 is a 260 amino acid (aa) protein with a 20 aa signal, a 173 aa extracellular domain, a 20 aa transmembrane domain, and a 47 aa cytoplasmic domain. The ligand for CD27 is CD70. CD70 is expressed on thymic stromal cells and a small subset of activated T cells. Additionally a subset of activated B cells express CD70. The CD27/CD70 interaction appears to be a weak costimulatory pathway involved in T cell and B cell immune response. CD27/CD70 interactions may be more involved in controlling the expansion phase of an immune response. This would be in contrast to B7/CD28 interactions, which are important for the activation phase of immune responses.

• References:

1. Camerini, D. et al. (1991) J. Immunol. 147:3165.

2. Loenen, W.A. et al. (1992) J. Immunol. 149:3937.

3. Lens, S.M.A. et al. (1998) Sem. Immunol. 10:491.

• Entrez Gene IDs:

  939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)

• Alternate Names:

  CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7