• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human NLRP10/Pynod/NALP10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) NALP1 (aa 1‑323), rhNALP1 (aa 1331‑1429), rhNALP2 (aa 1‑208), rhNALP3 (aa 3‑223), rhNALP3 (aa 540‑689), rhNALP5 (aa 469‑635), rhNOD1 (aa 830‑953), or rhNOD2 (aa 230‑450) is observed.    
   

• Source

  Monoclonal Mouse IgG    _2B Clone # 578502

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human NLRP10/Pynod/NALP10    
  Met386-Ile655    
  Accession # Q86W26

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.5 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NLRP10/Pynod/NALP10

NLRP10, also known as Pynod, NALP10, and NOD8, is a 75 kDa cytoplasmic protein that associates with the ASC component of inflammasomes. NLRP10 inhibits ASC‑dependent apoptosis, NFkB activation, and Caspase-1-dependent processing of IL-1 beta. Human NLRP10 contains one Pyrin domain (aa 1-96) and one NACHT domain (aa 167-484) but no leucine-rich repeats. Within aa 386-655, human NLRP10 shares approximately 50% aa sequence identity with mouse and rat NLRP10.

• Long Name:

  NLR Family, Pyrin Domain Containing 10

• Entrez Gene IDs:

  338322 (Human); 244202 (Mouse); 293426 (Rat)

• Alternate Names:

  CLR11.1; NACHT, leucine rich repeat and PYD containing 10; NALP10; NALP10Pynod; NLR family, pyrin domain containing 10; NLRP10; NOD8; NOD8Nucleotide-binding oligomerization domain protein 8; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 10; PAN5; Pynod; PYNODNACHT, LRR and PYD domains-containing protein 10