• Species Reactivity

  Human

• Specificity

  Detects HEK293 cells transfected with human CHRM3 in Cell-based ELISA    
   

• Source

  Monoclonal Mouse IgG    _2B Clone # 580011

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human CHRM3    
  Accession # P20309

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 µg/mL

  Immersion fixed paraffin-embedded sections of human pancreas 

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CHRM3

Muscarinic acetylcholine receptor M3 (CHRM3) is a 65 kDa 7-transmembrane glycoprotein that is predominantly expressed in the central nervous system, smooth muscle, and heart. CHRM3 mediates signal transduction in the autonomic nervous system in response to muscarinic agonists. Human CHRM3 shares 92% aa sequence identity with mouse and rat CHRM3.

• Long Name:

  Cholinergic Receptor Muscarinic 3

• Entrez Gene IDs:

  1131 (Human); 12671 (Mouse); 24260 (Rat)

• Alternate Names:

  cholinergic receptor, muscarinic 3; CHRM3; HM3; m3 muscarinic receptor; mAChR M3; muscarinic acetylcholine receptor M3